Coding

Part:BBa_K2562002

Designed by: Marina Takehana   Group: iGEM18_ASIJ_Tokyo   (2018-09-30)


E342K point mutation corrected through CRISPR technology in the SERPINA1 gene

The SERPINA1 gene coding for the Alpha-1 Antitrypsin protein is derived from Homo sapiens. We used CRISPR technology to cut out the faulty mutation and replace the E342K point mutation with its correct amino acid in the SERPINA1 gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/basic_part
Parameters
None